Assistant Professor (Stage III & Head)     Nov 2023-Present
 Department of Bioinformatics 
 St Aloysius (Deemed To Be) University
 Mangalore, India
 Academic lectures & Laboratory Supervisor
 Member BOS & BOE and other relevant committees
 Research Advisor to various Projects under Lifesciences

Teaching & Mentorship; 
 Biochemistry, Immunology, Computational Immunology and Immunomics, Therapeutics,
 Biologics & Proteomics 
 Immunophenotyping and Flowcytometry Bioinformatics

Trainee Researcher - Cancer Biology Laboratory May 2023-Oct 2023

Rajiv Gandhi Centre for Biotechnology (DBT), Trivandrum, India
• Anti-RBD S1 Monoclonal Antibody Development
• Transfection, FACS & Stable Cell line development
• Immunofluorescence & Immunohistochemistry (Diagnostic assay Standardisation)

Senior Scientific Officer Feb 2023-Nov 2023
Trophic Biosystems, Kochi, India
• Establishment of Cell-Culture facility & Assay system Development 
• Maintenance of Cell line (MCF-7) and Molecular facilities
• Project Design for the production of therapeutics & Enzymes

Post-Doctoral Researcher May 2022- Jan 2023
National Centre for Aquatic Animal Health
Cochin University of Science & Technology, 
Kochi, Kerala, India
• Primary Cell Culture for cell line development (Fish Kidney & Liver tissue)
• Skill development program on ‘Aquatic Pathogens Molecular Diagnosis’ funded by RUSA.
• Faculty- Bioinformatics & Immuno-informatics courses under M. Tech (Marine Biotechnology) 
program

Senior Scientific Officer Jan 2022– March 2022
(Spice HealthCare Pvt Ltd, Kerala, India) 
• Supervision of routine RT-PCR testing for COVID-19 & results validation 
• Internal Laboratory Quality Control as per ICMR guidelines
• Supervision of the Scientific Officers and Molecular Technicians.
• Supervision of protocols of Infection control & Biomedical Waste Management

Post-Doctoral Researcher July 2019- Oct 2021 
(NCAAH, Kochi, India)
• Collection of marine pathogenic proteins for antigen & peptide design.
• Development and optimization of synthetic peptide-based invitro & invivo assays
• Develop plan & proposal for the application of findings in further research.

Assistant Professor May 2015- July 2019 
DBT-Centre for Bioinformatics
AIMIT, St. Aloysius College, Mangalore, India
• Regular theoretical and practical sessions on Biochemistry, 
 Immunology and Bio-informatics areas
• Guidance of post-graduate dissertation on Biotechnology & Bioinformatics areas
• Member of Board of studies

Senior Research Fellow Feb 2013-Mar 2014 
(Karnataka Veterinary, Animal & Fisheries Sciences
University, Mangalore, India)
Project: Expression study of Noda virus gene for diagnostic development- National Agriculture Innovation 
Project-ICAR (Indian Council for Agriculture Research)
• Basic molecular and sequence analysis of Noda virus gene insert 
• Revival & Purification of plasmids for Noda viral protein extraction.

Senior Research Fellow July 2009-July 2012 
(Karnataka Veterinary, Animal & Fisheries Sciences
University, Mangalore, India)
Project: Cell culture and related works for Vibrio monoclonal antibodies production and characterization￾by RKVY (Rashtriya Krishi Vikas Yojana) Project of Government of India 
• Monoclonal Antibody Development against vibrio 
• Cell culture, MAb characterisation & Validation

Post-Graduate Lecturer July 2005- May 2009
 St Aloysius College, Mangalore, India 
 Regular theoretical & practical sessions of Bioinformatics and Biotechnology

• Industry Sponsored project of Expression of Antibody chains from hybridoma cell lines at Rajiv Gandhi Centre for Biotechnology during February 2023 to November 2023.
• Post Doctoral Researcher- RUSA Skill Development Program in Aquatic Animal Health, National Centre for Aquatic Animal Health, Kochi; May 2022 -Dec 2022.
• Post Doctoral Researcher- Institutional Network Project on Fish and Shell Fish Vaccine, National Centre for Aquatic Animal Health, Kochi, July 2019- Sep 2021.
• Research Officer-COVID Lab, Central University of Kerala, Molecular techniques for COVID Diagnosis and training, Oct 2021- Jan 2022.

• Shine, P. V., Shankar, K. M., Abhiman, B., Sudheer, N. S., & Patil, R. (2020). Epitope mapping of the White Spot Syndrome Virus (WSSV) VP28 monoclonal antibody through combined in silico and in vitro analysis reveals the potential antibody binding site. Molecular and Cellular Probes, 101508.
• Predictive Epitope and Validation on White Spot Syndrome Virus VP28: Insights for Candidate Vaccine and Comparative Efficacy with Synthetic Peptides (Under Review).
• Comprehensive Analysis of Antibody Cloning, Epitope Prediction, and Antigen Design for White Spot Syndrome Virus through Molecular Dynamics (Under Communication).
• Ashwini S, Shine P. Varkey, Manjula Shantaram. “In silico docking of polyphenolic compounds against caspase 3-HeLa cell line protein”. Journal of Drug Development Research, 2017; 9(3): 28-32.
• Computational Mapping of Interaction Sites of Synthetic Antibody Clone P109D9 on VP28 Protein (Conference Proceedings).

Exploring the Interaction Landscape of WSSV VP28-Antibody: Computational and Molecular Perspectives
Shine P Varkey†, Shankar K M ‡, Abhiksha Shetty†

 India-Netherlands Collaborative Program, Conference on Immune Biology and Precision Medicine: Deciphering Cross-Talks through Multi-Omics. 

‘Computational Study to explore the Epitope Landscape of VP28 of White Spot Syndrome Virus: Implications in Diagnostic and Therapeutic Design’, February 2023, Symposium on “Accelerating Biology 2024: The Exascale Leap” February 6-8, C-DAC, Pune.

An Analysis on the epitope-paratope relationship of anti-VP28 antibodies: Implications in therapeutic development against the White Spot Syndrome Virus, Conference-Monoclonal Antibodies, an emerging era in biologicals, December 2022, Institute of Advanced Virology, Trivandrum.

Epitope-CDR H3 loop relationship of antibodies interacting with VP28 protein of White Spot Syndrome Virus– Invited lecture on Structural Bioinformatics ‘New Horizons and Challenges in Biotechnology and Bioinformatics’, CPCRI, Kasargod, January 2014.

Computational Mapping of Interaction Sites of Synthetic Antibody Clone P109D9 on VP28 Protein, Paper Presentation, Conference on ‘Innovative Research in Computations, St Aloysius College-March 2014.

Member- The Antibody Society (TABs- Post Doctoral Membership)

Collaborations;
Rajiv Gandhi Centre for Biotechnology Trivandrum, India 

Institute of Advanced Virology, Trivandrum &
AJ Institute of Medical Sciences, Mangalore, India

DBT Skill Vigyan faculty training Program (14 days)- "Phage applications 2024
 and antimicrobial resistance" at Amrita University, Kerala, India 

Industrial Training Program on ‘Advanced Bio-Programing’ 2007 
Sai’s Bio Science Research Institute, Chennai, India.

Post Graduate Diploma in Bioinformatics 2004-2005 
Integrated Biotechnology (CRO), Bangalore, India 

International symposium on Multi-Omics Approaches in Understanding Environmental and 
Lifestyle Influences on Health, 9th October 2024, Nitte University Centre for Science Education 
& Research, Nitte (Deemed to be University), India.
• 5 days Residential Bio-Entrepreneurship Advanced Certification course cum Bootcamp by 
Kerala Biotechnology Commission & BIIC, January 2023, MG University, Kottayam.
• National Conference & Hands on Workshop on ‘Knowledge Translation Research’, by The 
Kerala State Higher Education Council 16th & 17th December 2023, at Cochin University of 
Science & Technology, Kochi, India.
• Workshop on ‘Monoclonal Antibodies, an emerging era in biologicals; Principles of 
Production, applications in immunodiagnostics & therapeutics, Institute of Advanced 
Virology, Trivandrum, India.
• Organized three days national workshop on ‘Research to Commercialisation’, 24-26th
Nov,2022, BIIC, MG University, Kottayam, India.
• 8th International Symposium on ‘Diseases in Asian Aquaculture’, 21-25 November 2011 by
Asian Fisheries Society, Mangalore, India.
• Workshop on ‘Bioinformatics Analysis using Accelrys GCG and SeqLab 3-4 Aug 2007, 
Chennai, India.